Hotspot

psaC-ndhE

SSC · 129782-130495 · 714 bp

Back to Ensete

Pi 0.046
MarkerSeek score 79.10
Primer available yes
Label psaC-ndhE

Feature Metadata

label_name
psaC-ndhE
feature_type
igs
region
SSC
length_bp
714
variable_sites
57
pi
0.046
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhE_p1 GAGTACGGGTTTTTGTCGGT TGGACCATTGGAGATGGAGT 50.0 50.0 58.1 58.0 1627-1734 bp 1.0 86.4
psaC-ndhE_p2 GCTGCGAGTTGTTTCATACG TGGACCATTGGAGATGGAGT 50.0 50.0 57.9 58.0 1547-1654 bp 1.0 86.4
psaC-ndhE_p3 TTTGACCGCTGTACATTGCT TGGACCATTGGAGATGGAGT 45.0 50.0 58.1 58.0 2067-2173 bp 1.0 86.2
psaC-ndhE_p4 ATCGATTCCCAGTCTCCAGT TGGACCATTGGAGATGGAGT 50.0 50.0 57.8 58.0 1940-2046 bp 1.0 85.9
psaC-ndhE_p5 AGTCCATCGATTCCCAGTCT TGGACCATTGGAGATGGAGT 50.0 50.0 57.8 58.0 1945-2022 bp 0.5 64.3