Hotspot

ccsA-ndhD

SSC · 127676-127908 · 233 bp

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Pi 0.0568
MarkerSeek score 78.30
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
233
variable_sites
22
pi
0.0568
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TGGGACCCCAAAGAAACTTG TTGCATTGCCAGGAATGAGT 50.0 45.0 57.9 58.1 796-811 bp 1.0 84.6
ccsA-ndhD_p2 TGGGACCCCAAAGAAACTTG AAAATGGGCGCATATGGACT 50.0 45.0 57.9 57.9 1190-1205 bp 1.0 83.2
ccsA-ndhD_p3 TGGGACCCCAAAGAAACTTG AAATGGGCGCATATGGACTT 50.0 45.0 57.9 57.9 1189-1204 bp 1.0 83.2
ccsA-ndhD_p4 TGGGACCCCAAAGAAACTTG GGATTCTGGACCACGAGAAC 50.0 55.0 57.9 58.0 588-603 bp 0.75 74.1
ccsA-ndhD_p5 TTGGACCATATTCGCGGTTT GGATTCTGGACCACGAGAAC 45.0 55.0 57.9 58.0 571 bp 0.25 49.0