Hotspot

rpl22-rps19

LSC · 87321-87535 · 215 bp

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Pi 0.0775
MarkerSeek score 80.00
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
LSC
length_bp
215
variable_sites
27
pi
0.0775
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 ACTTCTTTACCCGAACTTGAGT TTGCGAGACATGCAAGAAAC 40.9 45.0 57.3 57.3 288-349 bp 1.0 63.8
rpl22-rps19_p2 ACTTCTTTACCCGAACTTGAGTT TTGCGAGACATGCAAGAAAC 39.1 45.0 58.0 57.3 288-349 bp 1.0 61.0
rpl22-rps19_p3 ACTTCTTTACCCGAACTTGAGT GCGAGACATGCAAGAAACGA 40.9 50.0 57.3 59.2 286-347 bp 1.0 59.6
rpl22-rps19_p4 ACTTCTTTACCCGAACTTGAGT GCGAGACATGCAAGAAACG 40.9 52.6 57.3 57.7 286-347 bp 1.0 59.0
rpl22-rps19_p5 CTTCTTTACCCGAACTTGAGT TTGCGAGACATGCAAGAAAC 42.9 45.0 55.6 57.3 287-348 bp 1.0 57.7