Hotspot

accD-psaI

LSC · 61973-62265 · 293 bp

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Pi 0.0347
MarkerSeek score 63.80
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
293
variable_sites
19
pi
0.0347
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 CCACGGTTTCTTTCCCTTGA GAGGATAAGTTGGGATCTGTCA 50.0 45.5 58.0 57.0 356-657 bp 1.0 58.3
accD-psaI_p2 TCCACGGTTTCTTTCCCTTG GAGGATAAGTTGGGATCTGTCA 50.0 45.5 58.0 57.0 357-658 bp 1.0 58.3
accD-psaI_p3 CCACGGTTTCTTTCCCTTGA AGGATAAGTTGGGATCTGTCA 50.0 42.9 58.0 55.8 355-656 bp 1.0 56.5
accD-psaI_p4 TCCACGGTTTCTTTCCCTTG AGGATAAGTTGGGATCTGTCA 50.0 42.9 58.0 55.8 356-657 bp 1.0 56.5
accD-psaI_p5 CTCCACGGTTTCTTTCCCTT GAGGATAAGTTGGGATCTGTCA 50.0 45.5 57.7 57.0 358-659 bp 1.0 56.3