Hotspot

ndhC-trnV-UAC

LSC · 53299-54307 · 1009 bp

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Pi 0.0209
MarkerSeek score 67.90
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1009
variable_sites
38
pi
0.0209
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GCCAAGATAGGAATAACGCTTG AGAAGGTCTACGGTTCGAGT 45.5 50.0 57.5 57.8 1113-1548 bp 1.0 64.8
ndhC-trnV-UAC_p2 GCCAAGATAGGAATAACGCT AGAAGGTCTACGGTTCGAGT 45.0 50.0 55.3 57.8 1113-1548 bp 1.0 63.1
ndhC-trnV-UAC_p3 GCCAAGATAGGAATAACGCTTG AGCCCAATGCAACAAAATGC 45.5 45.0 57.5 58.5 1195-1630 bp 1.0 62.4
ndhC-trnV-UAC_p4 GCCAAGATAGGAATAACGCTTG GAAGGTCTACGGTTCGAGTC 45.5 55.0 57.5 57.5 1112-1547 bp 1.0 62.0
ndhC-trnV-UAC_p5 GCCAAGATAGGAATAACGCTT AGAAGGTCTACGGTTCGAGT 42.9 50.0 56.1 57.8 1113-1548 bp 1.0 61.5