Hotspot

trnE-UUC-trnT-GGU

LSC · 33340-34121 · 782 bp

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Pi 0.0213
MarkerSeek score 69.10
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
782
variable_sites
28
pi
0.0213
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 TCCTTTCGTAGTACCCCCAG GAACCGATGACTTACGCCTT 55.0 50.0 58.1 58.0 732-925 bp 1.0 85.4
trnE-UUC-trnT-GGU_p2 TCCTTTCGTAGTACCCCCAG CCATGGCGTTACTCTACCAC 55.0 55.0 58.1 58.1 711-904 bp 1.0 85.0
trnE-UUC-trnT-GGU_p3 TCCTTTCGTAGTACCCCCAG TGGCGTTACTCTACCACTGA 55.0 50.0 58.1 58.1 708-901 bp 1.0 84.8
trnE-UUC-trnT-GGU_p4 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 696-889 bp 1.0 84.5
trnE-UUC-trnT-GGU_p5 TCCTTTCGTAGTACCCCCAG TGGAAAAAGCCCCTTATCGG 55.0 50.0 58.1 57.9 756-949 bp 1.0 84.2