Hotspot

rps16-trnQ-UUG

LSC · 6117-7759 · 1643 bp

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Pi 0.0193
MarkerSeek score 67.30
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1643
variable_sites
57
pi
0.0193
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CAGCGGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1716-1773 bp 1.0 83.7
rps16-trnQ-UUG_p2 GCGGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1714-1771 bp 1.0 83.7
rps16-trnQ-UUG_p3 CAGCGGATCATGTCCTTCAA CCAAACAGGAAAAATGGGGC 50.0 50.0 58.0 57.8 1778-1835 bp 1.0 82.7
rps16-trnQ-UUG_p4 CAGCGGATCATGTCCTTCAA GCCAAACAGGAAAAATGGGG 50.0 50.0 58.0 57.8 1779-1836 bp 1.0 82.7
rps16-trnQ-UUG_p5 GCGGATCATGTCCTTCAAGT CCAAACAGGAAAAATGGGGC 50.0 50.0 58.0 57.8 1776-1833 bp 1.0 82.7