Hotspot

matK-tRNA-Lys

LSC · 3386-4241 · 856 bp

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Pi 0.0261
MarkerSeek score 68.20
Primer available yes
Label matK-tRNA-Lys

Feature Metadata

label_name
matK-tRNA-Lys
feature_type
igs
region
LSC
length_bp
856
variable_sites
40
pi
0.0261
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-tRNA-Lys_p1 TCTAAATATCCTTGTAATTCTTCCA AACGGTAGAGTACTCGGCTT 28.0 50.0 53.8 58.2 877-906 bp 1.0 47.2
matK-tRNA-Lys_p2 TCTAAATATCCTTGTAATTCTTCCA ACGGTAGAGTACTCGGCTTT 28.0 50.0 53.8 58.2 876-905 bp 1.0 47.2
matK-tRNA-Lys_p3 TTCTAAATATCCTTGTAATTCTTCCA AACGGTAGAGTACTCGGCTT 26.9 50.0 54.5 58.2 878-907 bp 1.0 47.2
matK-tRNA-Lys_p4 TTCTAAATATCCTTGTAATTCTTCCA ACGGTAGAGTACTCGGCTTT 26.9 50.0 54.5 58.2 877-906 bp 1.0 47.2
matK-tRNA-Lys_p5 TCTAAATATCCTTGTAATTCTTCCA GGGTTGCTAACTCAACGGTA 28.0 50.0 53.8 57.5 890-919 bp 1.0 47.1