Hotspot

ndhG

SSC · 107591-108121 · 531 bp

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Pi 0.00188

MarkerSeek score 44.70

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 1
pi: 0.00188
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	ACCATGTTTCGGCTTGTGAT	TTACATCAGAGCGTTTCCGG	45.0	50.0	58.1	58.0	1446 bp	1.0	79.6
ndhG_p2	ACCATGTTTCGGCTTGTGAT	TGCTTTGAGTCGGTTACCAG	45.0	50.0	58.1	57.8	1169 bp	1.0	78.4
ndhG_p3	ACCATGTTTCGGCTTGTGAT	GCTTTGAGTCGGTTACCAGT	45.0	50.0	58.1	57.8	1168 bp	1.0	78.4
ndhG_p4	ACCATGTTTCGGCTTGTGAT	GGGGGCGAATACACTTTGAA	45.0	50.0	58.1	58.2	1428 bp	1.0	78.3
ndhG_p5	GCTCTAACCATGTTTCGGCT	TTACATCAGAGCGTTTCCGG	50.0	50.0	58.3	58.0	1452 bp	1.0	78.3