Hotspot

atpF

LSC · 32486-33878 · 1393 bp

Back to Enneapogon

Pi 0.00649

MarkerSeek score 57.30

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1393
variable_sites: 9
pi: 0.00649
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	AAAAAGGGCGAGCGAAGTAA	CTCGAAGGGTTGCCATTCTT	45.0	50.0	58.1	58.2	1569-1605 bp	1.0	78.5
atpF_p2	AAAAAGGGCGAGCGAAGTAA	CGAAGGGTTGCCATTCTTGA	45.0	50.0	58.1	58.5	1567-1603 bp	1.0	76.1
atpF_p3	AAAAAGGGCGAGCGAAGTAA	TCGAAGGGTTGCCATTCTTG	45.0	50.0	58.1	58.5	1568-1604 bp	1.0	76.1
atpF_p4	CGAGCGAAGTAAGTGATCGA	CTCGAAGGGTTGCCATTCTT	50.0	50.0	57.5	58.2	1561-1597 bp	1.0	75.7
atpF_p5	AAAAGGGCGAGCGAAGTAAG	CTCGAAGGGTTGCCATTCTT	50.0	50.0	58.6	58.2	1568-1604 bp	1.0	74.9