Hotspot

rps18-rpl20

LSC · 64484-64721 · 238 bp

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Pi 0.0129
MarkerSeek score 62.30
Primer available yes
Label rps18-rpl20

Feature Metadata

label_name
rps18-rpl20
feature_type
igs
region
LSC
length_bp
238
variable_sites
3
pi
0.0129
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps18-rpl20_p1 CAACAATCGGAGCTTAAGTTCC ATGCTTGCACAAGTAGCTGT 45.5 45.0 57.7 58.1 338-344 bp 1.0 62.6
rps18-rpl20_p2 GGAGCTTAAGTTCCGATTGTTG ATGCTTGCACAAGTAGCTGT 45.5 45.0 57.7 58.1 330-336 bp 1.0 62.6
rps18-rpl20_p3 AAGAAACAACAATCGGAGCT ATGCTTGCACAAGTAGCTGT 40.0 45.0 55.6 58.1 344-350 bp 1.0 61.9
rps18-rpl20_p4 AGAAACAACAATCGGAGCTT ATGCTTGCACAAGTAGCTGT 40.0 45.0 55.6 58.1 343-349 bp 1.0 61.9
rps18-rpl20_p5 GGAGCTTAAGTTCCGATTGT ATGCTTGCACAAGTAGCTGT 45.0 45.0 55.5 58.1 330-336 bp 1.0 61.1