Hotspot

trnE-UUC-trnY-GUA

LSC · 14736-14796 · 61 bp

Back to Enneapogon

Pi 0.0328

MarkerSeek score 61.80

Primer available yes

Label trnE-UUC-trnY-GUA

Feature Metadata

label_name: trnE-UUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 61
variable_sites: 2
pi: 0.0328
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnY-GUA_p1	CTCTCTTTCAAGGAGGCAGC	CTAGATTTTTGGGCCGAGCT	55.0	50.0	58.3	58.0	199 bp	1.0	78.3
trnE-UUC-trnY-GUA_p2	TCGTCTAGTGGTTCAGGACA	CTAGATTTTTGGGCCGAGCT	50.0	50.0	57.7	58.0	220 bp	1.0	78.1
trnE-UUC-trnY-GUA_p3	CTCTCTTTCAAGGAGGCAGC	TAGATTTTTGGGCCGAGCTG	55.0	50.0	58.3	58.2	198 bp	1.0	76.7
trnE-UUC-trnY-GUA_p4	TCGTCTAGTGGTTCAGGACA	TAGATTTTTGGGCCGAGCTG	50.0	50.0	57.7	58.2	219 bp	1.0	76.5
trnE-UUC-trnY-GUA_p5	CTCTCTTTCAAGGAGGCAGC	CCCTAGATTTTTGGGCCGAG	55.0	55.0	58.3	58.3	201 bp	1.0	76.1