Hotspot

trnG-UCC-trnT-GGU

LSC · 13103-14086 · 984 bp

Back to Enneapogon

Pi 0.00306

MarkerSeek score 60.90

Primer available yes

Label trnG-UCC-trnT-GGU

Feature Metadata

label_name: trnG-UCC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 984
variable_sites: 3
pi: 0.00306
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnT-GGU_p1	ACGAATCACACTTTTACCACT	ACCGATGACTTCTGTCTTGC	38.1	50.0	55.5	57.9	1135-1138 bp	1.0	51.5
trnG-UCC-trnT-GGU_p2	AGAACGAATCACACTTTTACCAC	ACCGATGACTTCTGTCTTGC	39.1	50.0	57.3	57.9	1138-1141 bp	1.0	50.5
trnG-UCC-trnT-GGU_p3	GAACGAATCACACTTTTACCACT	ACCGATGACTTCTGTCTTGC	39.1	50.0	57.3	57.9	1137-1140 bp	1.0	50.5
trnG-UCC-trnT-GGU_p4	AACGAATCACACTTTTACCACT	ACCGATGACTTCTGTCTTGC	36.4	50.0	56.2	57.9	1136-1139 bp	1.0	49.6
trnG-UCC-trnT-GGU_p5	TACCACTAAACTATACCCGC	ACCGATGACTTCTGTCTTGC	45.0	50.0	54.0	57.9	1121-1124 bp	1.0	47.7