Hotspot

accD

LSC · 53949-55901 · 1953 bp

Back to Enkianthus

Pi 0.0141

MarkerSeek score 54.20

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1953
variable_sites: 72
pi: 0.0141
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TGTCACCAAAGAAAACCCCA	AATGAGGGACGTGATCTTGC	45.0	50.0	57.5	58.0	2218-2482 bp	0.214286	49.1
accD_p2	TGTCACCAAAGAAAACCCCA	GCCGTAGCTAATCGAGTAGC	45.0	55.0	57.5	58.0	2263-2527 bp	0.214286	49.1
accD_p3	TGTCACCAAAGAAAACCCCA	GGGTCTCTCTACCAGGTGTT	45.0	55.0	57.5	58.1	2409-2673 bp	0.214286	48.8
accD_p4	TGTCACCAAAGAAAACCCCA	TTGGGTCTCTCTACCAGGTG	45.0	55.0	57.5	58.1	2411-2675 bp	0.214286	48.8
accD_p5	TGTCACCAAAGAAAACCCCA	GCTCTGACCGAGATCTTTGG	45.0	55.0	57.5	58.1	2347-2611 bp	0.214286	48.8