Hotspot

ycf1-trnN-GUU

IRa · 133750-135581 · 1832 bp

Back to Enkianthus

Pi 0.0222

MarkerSeek score 59.00

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: IRa
length_bp: 1832
variable_sites: 93
pi: 0.0222
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	ACGTCTCAACAAATCTTTTAGA	AGGATTCGAACCTACGACCA	31.8	50.0	53.7	58.2	1083-2722 bp	1.0	48.2
ycf1-trnN-GUU_p2	ACGTCTCAACAAATCTTTTAGAATCA	AGGATTCGAACCTACGACCA	30.8	50.0	57.6	58.2	1083-2722 bp	1.0	48.2
ycf1-trnN-GUU_p3	ACGTCTCAACAAATCTTTTAGA	AATCAGTTAACAGCCGACCG	31.8	50.0	53.7	58.3	1064-2703 bp	1.0	48.2
ycf1-trnN-GUU_p4	ACGTCTCAACAAATCTTTTAGA	CCCCAAGTAGGATTCGAACC	31.8	55.0	53.7	57.7	1091-2730 bp	1.0	48.2
ycf1-trnN-GUU_p5	ACGTCTCAACAAATCTTTTAGAA	AGGATTCGAACCTACGACCA	30.4	50.0	54.5	58.2	1083-2722 bp	1.0	48.2