Hotspot

psbE-petL

LSC · 68130-69408 · 1279 bp

Back to Enkianthus

Pi 0.00702

MarkerSeek score 57.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1279
variable_sites: 27
pi: 0.00702
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	ACCAATAAACAGAGCGGAGG	50.0	50.0	57.9	57.6	1425-1476 bp	1.0	80.6
psbE-petL_p2	CAACCCGCAATGAATAGGGA	CCAATAAACAGAGCGGAGGT	50.0	50.0	57.9	57.6	1424-1475 bp	1.0	80.6
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	ACCAATAAACAGAGCGGAGG	50.0	50.0	57.8	57.6	1348-1399 bp	1.0	80.0
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	CCAATAAACAGAGCGGAGGT	50.0	50.0	57.8	57.6	1347-1398 bp	1.0	80.0
psbE-petL_p5	ACGAATAACCAACCCGCAAT	CCAATAAACAGAGCGGAGGT	45.0	50.0	58.2	57.6	1433-1484 bp	1.0	79.6