Hotspot

ndhC-accD

LSC · 52493-53948 · 1456 bp

Back to Enkianthus

Pi 0.0111
MarkerSeek score 59.70
Primer available yes
Label ndhC-accD

Feature Metadata

label_name
ndhC-accD
feature_type
igs
region
LSC
length_bp
1456
variable_sites
50
pi
0.0111
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-accD_p1 TGCCAAAATAGGAATAAGGCTTG AGGGCGCATTCGATCTAATC 39.1 50.0 57.4 57.9 1579-1642 bp 1.0 56.3
ndhC-accD_p2 TGCCAAAATAGGAATAAGGCTTG GATAAGGGCGCATTCGATCT 39.1 50.0 57.4 57.9 1583-1646 bp 1.0 56.3
ndhC-accD_p3 TGCCAAAATAGGAATAAGGCT AGGGCGCATTCGATCTAATC 38.1 50.0 55.2 57.9 1579-1642 bp 1.0 54.8
ndhC-accD_p4 TGCCAAAATAGGAATAAGGCT GATAAGGGCGCATTCGATCT 38.1 50.0 55.2 57.9 1583-1646 bp 1.0 54.8
ndhC-accD_p5 GCCAAAATAGGAATAAGGCTTGA AGGGCGCATTCGATCTAATC 39.1 50.0 57.1 57.9 1578-1641 bp 1.0 54.3