Hotspot

trnC-GCA-petN

LSC · 29327-29930 · 604 bp

Back to Enkianthus

Pi 0.00951

MarkerSeek score 57.80

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 604
variable_sites: 20
pi: 0.00951
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GGGTGTCGCCTAATCAACAA	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.2	58.2	637-652 bp	1.0	82.3
trnC-GCA-petN_p2	TTGTATCCTTTTGGCGGCAT	AGCCCAAGCGAGACTTACTA	45.0	50.0	58.2	58.2	709-724 bp	1.0	82.0
trnC-GCA-petN_p3	GACTGCAACTCCTCTTTCCC	AGCCCAAGCGAGACTTACTA	55.0	50.0	58.2	58.2	669-684 bp	1.0	82.0
trnC-GCA-petN_p4	GGACTGCAACTCCTCTTTCC	AGCCCAAGCGAGACTTACTA	55.0	50.0	58.2	58.2	670-685 bp	1.0	82.0
trnC-GCA-petN_p5	GGGACTGCAACTCCTCTTTC	AGCCCAAGCGAGACTTACTA	55.0	50.0	58.2	58.2	671-686 bp	1.0	82.0