Hotspot

rps16-trnQ-UUG

LSC · 6602-8027 · 1426 bp

Back to Enkianthus

Pi 0.00708

MarkerSeek score 61.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1426
variable_sites: 40
pi: 0.00708
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AACGGATCGTGTCCTTCAAG	AAGGTTCGAATCCTTCCGTC	50.0	50.0	57.9	57.6	1473-1523 bp	1.0	82.4
rps16-trnQ-UUG_p2	AACGGATCGTGTCCTTCAAG	TTGGTCCCGTTATTCGAAGG	50.0	50.0	57.9	57.6	1489-1539 bp	1.0	82.3
rps16-trnQ-UUG_p3	AACGGATCGTGTCCTTCAAG	GGTCCCGTTATTCGAAGGTT	50.0	50.0	57.9	57.6	1487-1537 bp	1.0	82.3
rps16-trnQ-UUG_p4	CAACGGATCGTGTCCTTCAA	AAGGTTCGAATCCTTCCGTC	50.0	50.0	58.2	57.6	1474-1524 bp	1.0	81.4
rps16-trnQ-UUG_p5	CAACGGATCGTGTCCTTCAA	TTGGTCCCGTTATTCGAAGG	50.0	50.0	58.2	57.6	1490-1540 bp	1.0	81.3