primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	AATCATAACCCTGGGCGAAC	TCGTATTCCAAGCTGAGCAC	20	20	50.0	50.0	57.9	58.0	14.1	7.4	0.062927	125	831	1265-1311	1.000000	86.0
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	AATCATAACCCTGGGCGAAC	ATTCCAAGCTGAGCACAACA	20	20	50.0	45.0	57.9	58.0	14.1	7.4	0.067309	125	831	1261-1307	1.000000	86.0
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCATAACCCTGGGCGAAC	TTCCAAGCTGAGCACAACAT	20	20	50.0	45.0	57.9	58.0	14.1	7.4	0.067309	125	831	1260-1306	1.000000	86.0
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	AATCATAACCCTGGGCGAAC	CTGATGGGATGCCTCTAGGA	20	20	50.0	55.0	57.9	58.0	14.1	4.3	0.074044	125	831	1310-1356	1.000000	85.9
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	AATCATAACCCTGGGCGAAC	TCTGATGGGATGCCTCTAGG	20	20	50.0	55.0	57.9	58.0	14.1	4.3	0.074044	125	831	1311-1357	1.000000	85.9
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CGAATCCCTTGCTTCATCCA	AGGCGGAGATTTTTAAGGAAGT	20	22	50.0	40.9	58.0	57.7	0.0	0.0	2.345245	4607	5475	920-986	1.000000	67.1
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	ACGAATCCCTTGCTTCATCC	AGGCGGAGATTTTTAAGGAAGT	20	22	50.0	40.9	58.0	57.7	0.0	0.0	2.346682	4607	5475	921-987	1.000000	67.1
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	ATGGACGAATCCCTTGCTTC	AGGCGGAGATTTTTAAGGAAGT	20	22	50.0	40.9	58.0	57.7	0.0	0.0	2.346682	4607	5475	925-991	1.000000	67.1
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	GGACGAATCCCTTGCTTCAT	AGGCGGAGATTTTTAAGGAAGT	20	22	50.0	40.9	58.0	57.7	0.0	0.0	2.346682	4607	5475	923-989	1.000000	67.1
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	AGGCGGAGATTTTTAAGGAAGT	20	22	50.0	40.9	58.2	57.7	20.3	0.0	2.470979	4607	5475	871-937	1.000000	66.2
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AACGGATCGTGTCCTTCAAG	AAGGTTCGAATCCTTCCGTC	20	20	50.0	50.0	57.9	57.6	7.8	19.8	0.462011	6602	8027	1473-1523	1.000000	82.4
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AACGGATCGTGTCCTTCAAG	TTGGTCCCGTTATTCGAAGG	20	20	50.0	50.0	57.9	57.6	7.8	3.3	0.470248	6602	8027	1489-1539	1.000000	82.3
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AACGGATCGTGTCCTTCAAG	GGTCCCGTTATTCGAAGGTT	20	20	50.0	50.0	57.9	57.6	7.8	3.3	0.471902	6602	8027	1487-1537	1.000000	82.3
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CAACGGATCGTGTCCTTCAA	AAGGTTCGAATCCTTCCGTC	20	20	50.0	50.0	58.2	57.6	7.8	19.8	0.590970	6602	8027	1474-1524	1.000000	81.4
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	CAACGGATCGTGTCCTTCAA	TTGGTCCCGTTATTCGAAGG	20	20	50.0	50.0	58.2	57.6	7.8	3.3	0.599207	6602	8027	1490-1540	1.000000	81.3
trnC-GCA-petN_p1	trnC-GCA-petN	1	GGGTGTCGCCTAATCAACAA	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.2	58.2	1.7	0.0	0.335274	29327	29930	637-652	1.000000	82.3
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTGTATCCTTTTGGCGGCAT	AGCCCAAGCGAGACTTACTA	20	20	45.0	50.0	58.2	58.2	14.3	0.0	0.305342	29327	29930	709-724	1.000000	82.0
trnC-GCA-petN_p3	trnC-GCA-petN	3	GACTGCAACTCCTCTTTCCC	AGCCCAAGCGAGACTTACTA	20	20	55.0	50.0	58.2	58.2	0.0	0.0	0.336555	29327	29930	669-684	1.000000	82.0
trnC-GCA-petN_p4	trnC-GCA-petN	4	GGACTGCAACTCCTCTTTCC	AGCCCAAGCGAGACTTACTA	20	20	55.0	50.0	58.2	58.2	3.2	0.0	0.336555	29327	29930	670-685	1.000000	82.0
trnC-GCA-petN_p5	trnC-GCA-petN	5	GGGACTGCAACTCCTCTTTC	AGCCCAAGCGAGACTTACTA	20	20	55.0	50.0	58.2	58.2	9.5	0.0	0.336555	29327	29930	671-686	1.000000	82.0
petN-psbM_p1	petN-psbM	1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	58.2	57.6	0.0	0.0	1.558772	30021	31241	1383-1406	1.000000	69.5
petN-psbM_p2	petN-psbM	2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.6	57.6	0.0	0.0	1.758286	30021	31241	1322-1345	1.000000	68.0
petN-psbM_p3	petN-psbM	3	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTC	20	20	50.0	45.0	58.2	56.1	0.0	0.0	2.081320	30021	31241	1383-1406	1.000000	65.3
petN-psbM_p4	petN-psbM	4	GGGGACAGAATTCACATGGA	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.2	57.6	8.4	0.0	2.214368	30021	31241	1405-1428	1.000000	64.3
petN-psbM_p5	petN-psbM	5	AGGGGACAGAATTCACATGG	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.2	57.6	8.4	0.0	2.222229	30021	31241	1406-1429	1.000000	64.2
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	20	20	55.0	45.0	58.1	58.2	7.6	0.0	0.310137	33521	34988	1617-1637	1.000000	78.8
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.332292	33521	34988	1620-1640	1.000000	78.6
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GTGGTAGAGTAACGCCATGG	CAAAAACGAAACGGTCCCTC	20	20	55.0	50.0	58.1	57.6	7.6	0.0	0.458613	33521	34988	1616-1636	1.000000	77.6
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GTGGTAGAGTAACGCCATGG	AGAGCAATAAACCCGACCAG	20	20	55.0	50.0	58.1	57.6	7.6	0.0	0.467319	33521	34988	1640-1660	1.000000	77.5
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	TCAGTGGTAGAGTAACGCCA	CAAAAACGAAACGGTCCCTC	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.480767	33521	34988	1619-1639	1.000000	77.4
ndhC-accD_p1	ndhC-accD	1	TGCCAAAATAGGAATAAGGCTTG	AGGGCGCATTCGATCTAATC	23	20	39.1	50.0	57.4	57.9	10.7	10.8	3.682890	52493	53948	1579-1642	1.000000	56.3
ndhC-accD_p2	ndhC-accD	2	TGCCAAAATAGGAATAAGGCTTG	GATAAGGGCGCATTCGATCT	23	20	39.1	50.0	57.4	57.9	10.7	10.8	3.682890	52493	53948	1583-1646	1.000000	56.3
ndhC-accD_p3	ndhC-accD	3	TGCCAAAATAGGAATAAGGCT	AGGGCGCATTCGATCTAATC	21	20	38.1	50.0	55.2	57.9	0.0	10.8	3.867678	52493	53948	1579-1642	1.000000	54.8
ndhC-accD_p4	ndhC-accD	4	TGCCAAAATAGGAATAAGGCT	GATAAGGGCGCATTCGATCT	21	20	38.1	50.0	55.2	57.9	0.0	10.8	3.867678	52493	53948	1583-1646	1.000000	54.8
ndhC-accD_p5	ndhC-accD	5	GCCAAAATAGGAATAAGGCTTGA	AGGGCGCATTCGATCTAATC	23	20	39.1	50.0	57.1	57.9	10.7	10.8	3.938337	52493	53948	1578-1641	1.000000	54.3
accD_p1	accD	1	TGTCACCAAAGAAAACCCCA	AATGAGGGACGTGATCTTGC	20	20	45.0	50.0	57.5	58.0	0.0	0.0	0.563880	53949	55901	2218-2482	0.214286	49.1
accD_p2	accD	2	TGTCACCAAAGAAAACCCCA	GCCGTAGCTAATCGAGTAGC	20	20	45.0	55.0	57.5	58.0	0.0	31.7	0.567620	53949	55901	2263-2527	0.214286	49.1
accD_p3	accD	3	TGTCACCAAAGAAAACCCCA	GGGTCTCTCTACCAGGTGTT	20	20	45.0	55.0	57.5	58.1	0.0	0.8	0.594062	53949	55901	2409-2673	0.214286	48.8
accD_p4	accD	4	TGTCACCAAAGAAAACCCCA	TTGGGTCTCTCTACCAGGTG	20	20	45.0	55.0	57.5	58.1	0.0	0.8	0.595457	53949	55901	2411-2675	0.214286	48.8
accD_p5	accD	5	TGTCACCAAAGAAAACCCCA	GCTCTGACCGAGATCTTTGG	20	20	45.0	55.0	57.5	58.1	0.0	12.2	0.600795	53949	55901	2347-2611	0.214286	48.8
rbcL-atpB_p1	rbcL-atpB	1	GTAAGTCCATCGGTCCACAC	TTTTCCTCCACGGCGATAAG	20	20	55.0	50.0	58.0	58.0	0.0	16.6	0.024512	57634	58860	1823-2049	1.000000	88.3
rbcL-atpB_p2	rbcL-atpB	2	GTAAGTCCATCGGTCCACAC	CGGTTCATTCATCTGACCGT	20	20	55.0	50.0	58.0	58.0	0.0	5.2	0.016116	57634	58860	2048-2274	1.000000	87.9
rbcL-atpB_p3	rbcL-atpB	3	CGGTCTCTCCAACGCATAAA	TTTTCCTCCACGGCGATAAG	20	20	50.0	50.0	58.0	58.0	0.0	16.6	0.023125	57634	58860	2249-2475	1.000000	87.6
rbcL-atpB_p4	rbcL-atpB	4	GGGGACGACCATACTTGTTC	CGGTTCATTCATCTGACCGT	20	20	55.0	50.0	58.0	58.0	6.2	5.2	0.030093	57634	58860	2329-2555	1.000000	87.5
rbcL-atpB_p5	rbcL-atpB	5	CGGTCTCTCCAACGCATAAA	CGGTTCATTCATCTGACCGT	20	20	50.0	50.0	58.0	58.0	0.0	5.2	0.014728	57634	58860	2474-2700	1.000000	87.4
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	ACCAATAAACAGAGCGGAGG	20	20	50.0	50.0	57.9	57.6	6.4	0.0	0.454586	68130	69408	1425-1476	1.000000	80.6
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	CCAATAAACAGAGCGGAGGT	20	20	50.0	50.0	57.9	57.6	6.4	0.0	0.454586	68130	69408	1424-1475	1.000000	80.6
psbE-petL_p3	psbE-petL	3	AGAACGTTCTCCTGTGCTTC	ACCAATAAACAGAGCGGAGG	20	20	50.0	50.0	57.8	57.6	24.9	0.0	0.559595	68130	69408	1348-1399	1.000000	80.0
psbE-petL_p4	psbE-petL	4	AGAACGTTCTCCTGTGCTTC	CCAATAAACAGAGCGGAGGT	20	20	50.0	50.0	57.8	57.6	24.9	0.0	0.559595	68130	69408	1347-1398	1.000000	80.0
psbE-petL_p5	psbE-petL	5	ACGAATAACCAACCCGCAAT	CCAATAAACAGAGCGGAGGT	20	20	45.0	50.0	58.2	57.6	0.0	0.0	0.574273	68130	69408	1433-1484	1.000000	79.6
trnN-GUU-ndhF_p1	trnN-GUU-ndhF	1	AGGATTCGAACCTACGACCA	GCGGGGTCGTATTTCTTCTT	20	20	50.0	50.0	58.2	58.0	15.0	0.0	0.176579	112381	113554	1203-1476	1.000000	89.8
trnN-GUU-ndhF_p2	trnN-GUU-ndhF	2	AATCAGTTAACAGCCGACCG	GCGGGGTCGTATTTCTTCTT	20	20	50.0	50.0	58.3	58.0	8.6	0.0	0.297936	112381	113554	1184-1457	1.000000	88.9
trnN-GUU-ndhF_p3	trnN-GUU-ndhF	3	CCCCAAGTAGGATTCGAACC	GCGGGGTCGTATTTCTTCTT	20	20	55.0	50.0	57.7	58.0	0.0	0.0	0.347148	112381	113554	1211-1484	1.000000	88.4
trnN-GUU-ndhF_p4	trnN-GUU-ndhF	4	CGAACCTACGACCAATCAGT	GCGGGGTCGTATTTCTTCTT	20	20	50.0	50.0	57.4	58.0	0.0	0.0	0.669279	112381	113554	1197-1470	1.000000	85.9
trnN-GUU-ndhF_p5	trnN-GUU-ndhF	5	GGATTCGAACCTACGACCAA	GCGGGGTCGTATTTCTTCTT	20	20	50.0	50.0	57.3	58.0	0.0	0.0	0.680136	112381	113554	1202-1475	1.000000	85.8
ndhF-rpl32_p1	ndhF-rpl32	1	TGGAAAAAGAAGAAGGCCCA	AGCTGCCCAATATCCTTTCC	20	20	45.0	50.0	57.2	57.6	0.0	0.0	1.137347	115796	116704	1044-1083	1.000000	77.1
ndhF-rpl32_p2	ndhF-rpl32	2	TGGAAAAAGAAGAAGGCCCA	GCTGCCCAATATCCTTTCCT	20	20	45.0	50.0	57.2	57.6	0.0	0.0	1.137347	115796	116704	1043-1082	1.000000	77.1
ndhF-rpl32_p3	ndhF-rpl32	3	ACAAAAGGTATGATCCACGCA	GCTGCCCAATATCCTTTCCT	21	20	42.9	50.0	57.9	57.6	0.0	0.0	1.437949	115796	116704	994-1033	1.000000	75.0
ndhF-rpl32_p4	ndhF-rpl32	4	TTGGAAAAAGAAGAAGGCCCA	AGCTGCCCAATATCCTTTCC	21	20	42.9	50.0	58.0	57.6	0.0	0.0	1.410910	115796	116704	1045-1084	1.000000	74.9
ndhF-rpl32_p5	ndhF-rpl32	5	TTGGAAAAAGAAGAAGGCCCA	GCTGCCCAATATCCTTTCCT	21	20	42.9	50.0	58.0	57.6	0.0	0.0	1.410910	115796	116704	1044-1083	1.000000	74.9
ycf1_p1	ycf1	1	TTTCTCTTTCCGCTATGCCC	CACGGTTGATGGCGTTTATG	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.210980	128638	128757	570-1053	1.000000	90.3
ycf1_p2	ycf1	2	TTTCTCTTTCCGCTATGCCC	ACGGTTGATGGCGTTTATGT	20	20	50.0	45.0	58.0	58.2	0.0	0.0	0.222312	128638	128757	569-1052	1.000000	90.2
ycf1_p3	ycf1	3	TTTCTCTTTCCGCTATGCCC	GCAACTGCCTGTAAAGCAAC	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.267430	128638	128757	686-1169	1.000000	89.9
ycf1_p4	ycf1	4	TTTCTCTTTCCGCTATGCCC	TCCAGGAGCCAAAAATGCTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.052397	128638	128757	1071-1551	0.642857	76.0
ycf1_p5	ycf1	5	TGGGTCAGTGTGTTACTCGA	TCCAGGAGCCAAAAATGCTT	20	20	50.0	45.0	58.3	58.0	0.0	0.0	0.319166	128638	128757	1135-1615	0.642857	73.5
ycf1-trnN-GUU_p1	ycf1-trnN-GUU	1	ACGTCTCAACAAATCTTTTAGA	AGGATTCGAACCTACGACCA	22	20	31.8	50.0	53.7	58.2	0.0	15.0	6.422681	133750	135581	1083-2722	1.000000	48.2
ycf1-trnN-GUU_p2	ycf1-trnN-GUU	2	ACGTCTCAACAAATCTTTTAGAATCA	AGGATTCGAACCTACGACCA	26	20	30.8	50.0	57.6	58.2	0.0	15.0	6.534804	133750	135581	1083-2722	1.000000	48.2
ycf1-trnN-GUU_p3	ycf1-trnN-GUU	3	ACGTCTCAACAAATCTTTTAGA	AATCAGTTAACAGCCGACCG	22	20	31.8	50.0	53.7	58.3	0.0	8.6	6.544038	133750	135581	1064-2703	1.000000	48.2
ycf1-trnN-GUU_p4	ycf1-trnN-GUU	4	ACGTCTCAACAAATCTTTTAGA	CCCCAAGTAGGATTCGAACC	22	20	31.8	55.0	53.7	57.7	0.0	0.0	6.593249	133750	135581	1091-2730	1.000000	48.2
ycf1-trnN-GUU_p5	ycf1-trnN-GUU	5	ACGTCTCAACAAATCTTTTAGAA	AGGATTCGAACCTACGACCA	23	20	30.4	50.0	54.5	58.2	0.0	15.0	6.621663	133750	135581	1083-2722	1.000000	48.2
ycf2-trnI-CAU_p1	ycf2-trnI-CAU	1	TCCCACGAAATTGAATTGGGT	TGAACCTACGAATTCGCCAA	21	20	42.9	45.0	58.1	57.5	11.4	27.0	1.576034	157581	157796	391-473	1.000000	70.8
ycf2-trnI-CAU_p2	ycf2-trnI-CAU	2	TCCCACGAAATTGAATTGGGT	TTGAACCTACGAATTCGCCA	21	20	42.9	45.0	58.1	57.5	11.4	27.0	1.576034	157581	157796	392-474	1.000000	70.8
ycf2-trnI-CAU_p3	ycf2-trnI-CAU	3	TCCCACGAAATTGAATTGGGT	TATTTGAGGGTCCCATTGGC	21	20	42.9	50.0	58.1	57.5	11.4	11.6	1.569574	157581	157796	428-510	1.000000	70.6
ycf2-trnI-CAU_p4	ycf2-trnI-CAU	4	CCACGAAATTGAATTGGGTCC	TATTTGAGGGTCCCATTGGC	21	20	47.6	50.0	57.8	57.5	0.0	11.6	1.683344	157581	157796	426-508	1.000000	69.7
ycf2-trnI-CAU_p5	ycf2-trnI-CAU	5	CCCACGAAATTGAATTGGGTC	TATTTGAGGGTCCCATTGGC	21	20	47.6	50.0	57.8	57.5	4.8	11.6	1.683344	157581	157796	427-509	1.000000	69.7