Hotspot

ndhF-rpl32

SSC · 118986-119929 · 944 bp

Back to Engelhardia

Pi 0.0122
MarkerSeek score 50.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
944
variable_sites
21
pi
0.0122
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.77
markerseek_score
50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 ACTGGAATGAAAGGTATGATCCA CTACCCAATACCCCTTCCCT 39.1 55.0 57.3 57.8 859-1088 bp 1.0 54.4
ndhF-rpl32_p2 ACTGGAATGAAAGGTATGATCCA ACCCAATACCCCTTCCCTTT 39.1 50.0 57.3 58.2 857-1086 bp 1.0 54.3
ndhF-rpl32_p3 ACTGGAATGAAAGGTATGATCCA CCAATACCCCTTCCCTTTCC 39.1 55.0 57.3 57.5 855-1084 bp 1.0 52.4
ndhF-rpl32_p4 ACTGGAATGAAAGGTATGATCCA CCCAATACCCCTTCCCTTTC 39.1 55.0 57.3 57.5 856-1085 bp 1.0 52.4
ndhF-rpl32_p5 AACTGGAATGAAAGGTATGATCCA CTACCCAATACCCCTTCCCT 37.5 55.0 57.9 57.8 860-1089 bp 1.0 51.5