Hotspot

trnN-GUU-ndhF

IRb · 115005-116741 · 1737 bp

Back to Engelhardia

Pi 0.0132

MarkerSeek score 47.70

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 1737
variable_sites: 37
pi: 0.0132
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 47.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	TGAACCTACGACCAGTCAGT	TCGTATCCCCATGAATCGGA	50.0	50.0	58.0	58.0	2027-2417 bp	1.0	85.7
trnN-GUU-ndhF_p2	AAGACAGAGGGAACGGGTTA	TCGTATCCCCATGAATCGGA	50.0	50.0	58.0	58.0	2127-2517 bp	1.0	85.7
trnN-GUU-ndhF_p3	TGAACCTACGACCAGTCAGT	ACTTTGTTCGTTGGAGCCAT	50.0	45.0	58.0	58.0	1967-2357 bp	1.0	85.6
trnN-GUU-ndhF_p4	AAGACAGAGGGAACGGGTTA	ACTTTGTTCGTTGGAGCCAT	50.0	45.0	58.0	58.0	2067-2457 bp	1.0	85.6
trnN-GUU-ndhF_p5	GGCAGAATAGCAGAGCAAGT	TCGTATCCCCATGAATCGGA	50.0	50.0	58.0	58.0	2424-2814 bp	1.0	85.4