Hotspot

rps19-rpl2

IRb · 90486-90569 · 84 bp

Back to Engelhardia

Pi 0.0102

MarkerSeek score 52.60

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 84
variable_sites: 2
pi: 0.0102
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	AGATGCTCGGGACCAAGTTA	TTGATTCTTCGTCGCCGTAG	50.0	50.0	58.4	58.1	209-218 bp	1.0	79.1
rps19-rpl2_p2	TAGATGCTCGGGACCAAGTT	TTGATTCTTCGTCGCCGTAG	50.0	50.0	58.4	58.1	210-219 bp	1.0	79.1
rps19-rpl2_p3	AGATGCTCGGGACCAAGTTA	GGGCCCCAATTGGTAGAAAA	50.0	50.0	58.4	58.1	301-310 bp	1.0	78.4
rps19-rpl2_p4	TAGATGCTCGGGACCAAGTT	GGGCCCCAATTGGTAGAAAA	50.0	50.0	58.4	58.1	302-311 bp	1.0	78.4
rps19-rpl2_p5	AGATGCTCGGGACCAAGTTA	TTGGGGTTATCCTGCACTTG	50.0	50.0	58.4	57.8	267-276 bp	1.0	77.5