Hotspot

trnW-CCA-trnP-UGG

LSC · 72443-72643 · 201 bp

Back to Engelhardia

Pi 0.0204

MarkerSeek score 52.80

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 201
variable_sites: 8
pi: 0.0204
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	GTTTTGGAGACCCACGTTCT	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.0	58.1	265-270 bp	1.0	84.2
trnW-CCA-trnP-UGG_p2	GTTTTGGAGACCCACGTTCT	CACGGGTTCAAATCCTGTCA	50.0	50.0	58.0	58.1	263-268 bp	1.0	84.2
trnW-CCA-trnP-UGG_p3	GTTTTGGAGACCCACGTTCT	GGGTACAAAATGTCACGGGT	50.0	50.0	58.0	58.1	276-281 bp	1.0	84.1
trnW-CCA-trnP-UGG_p4	GTTTTGGAGACCCACGTTCT	TGGGTACAAAATGTCACGGG	50.0	50.0	58.0	58.1	277-282 bp	1.0	84.0
trnW-CCA-trnP-UGG_p5	GTTTTGGAGACCCACGTTCT	ATGACACTGTCGACCAATGG	50.0	50.0	58.0	57.9	333-338 bp	1.0	83.2