Hotspot

petL-petG

LSC · 71946-72123 · 178 bp

Back to Engelhardia

Pi 0.0112

MarkerSeek score 51.00

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 178
variable_sites: 4
pi: 0.0112
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	TCGGTTTTCTACTAGCGGTT	CAACTGATCACCACGTCTGT	45.0	50.0	56.6	57.8	351-358 bp	1.0	69.8
petL-petG_p2	TTCGGTTTTCTACTAGCGGT	CAACTGATCACCACGTCTGT	45.0	50.0	56.6	57.8	352-359 bp	1.0	69.8
petL-petG_p3	TCGGTTTTCTACTAGCGGTTT	CAACTGATCACCACGTCTGT	42.9	50.0	57.3	57.8	351-358 bp	1.0	67.7
petL-petG_p4	TTCGGTTTTCTACTAGCGGTT	CAACTGATCACCACGTCTGT	42.9	50.0	57.3	57.8	352-359 bp	1.0	67.7
petL-petG_p5	TTTCGGTTTTCTACTAGCGGT	CAACTGATCACCACGTCTGT	42.9	50.0	57.3	57.8	353-360 bp	1.0	67.7