Hotspot

trnF-GAA-ndhJ

LSC · 53387-54173 · 787 bp

Back to Engelhardia

Pi 0.0208

MarkerSeek score 55.60

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 787
variable_sites: 35
pi: 0.0208
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	AATGCCGGAAAGCTGGATAG	50.0	50.0	58.1	58.0	903-1005 bp	1.0	83.5
trnF-GAA-ndhJ_p2	TGATACGCCAGAAATGGTCG	AATGCCGGAAAGCTGGATAG	50.0	50.0	58.1	58.0	958-1060 bp	1.0	83.2
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	GAAAGCTGGATAGGATGGCC	50.0	55.0	58.1	58.1	896-998 bp	1.0	83.0
trnF-GAA-ndhJ_p4	CCAGAAATGGTCGGGATAGC	AATGCCGGAAAGCTGGATAG	55.0	50.0	58.1	58.0	951-1053 bp	1.0	82.8
trnF-GAA-ndhJ_p5	GCCAGAAATGGTCGGGATAG	AATGCCGGAAAGCTGGATAG	55.0	50.0	58.1	58.0	952-1054 bp	1.0	82.8