Hotspot

psaA-ycf3

LSC · 46423-47240 · 818 bp

Back to Engelhardia

Pi 0.00619

MarkerSeek score 55.20

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 818
variable_sites: 10
pi: 0.00619
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TACTTCTGATTCCGGCGAAC	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.7	58.0	923-951 bp	1.0	80.4
psaA-ycf3_p2	TACTTCTGATTCCGGCGAAC	CAGAGGCTTGGTTCGATCAA	50.0	50.0	57.7	57.9	938-966 bp	1.0	79.8
psaA-ycf3_p3	TACTTCTGATTCCGGCGAAC	AAGATCACAAGGCGTTTCGA	50.0	45.0	57.7	57.8	849-877 bp	1.0	79.6
psaA-ycf3_p4	TACTTCTGATTCCGGCGAAC	AGATCACAAGGCGTTTCGAA	50.0	45.0	57.7	57.8	848-876 bp	1.0	79.6
psaA-ycf3_p5	TACTTCTGATTCCGGCGAAC	CGATCAAGCCGCTGAGTATT	50.0	50.0	57.7	58.1	925-953 bp	1.0	79.5