Hotspot

rps16-trnQ-UUG

LSC · 6437-7886 · 1450 bp

Back to Engelhardia

Pi 0.0133

MarkerSeek score 58.10

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1450
variable_sites: 36
pi: 0.0133
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AATGGCGGATGTCAGAATCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1038-1613 bp	1.0	85.1
rps16-trnQ-UUG_p2	CAGAATCCACAACCGACCAT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	1026-1601 bp	1.0	84.5
rps16-trnQ-UUG_p3	TCCACAACCGACCATATCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.1	58.1	1021-1596 bp	1.0	84.4
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	999-1574 bp	1.0	84.3
rps16-trnQ-UUG_p5	AATGGCGGATGTCAGAATCC	TGGCTAAGTGGTAAGGCAAC	50.0	50.0	58.0	57.8	1079-1654 bp	1.0	84.1