Hotspot

atpF

LSC · 13766-15039 · 1274 bp

Back to Endiandra

Pi 0.00118

MarkerSeek score 47.20

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1274
variable_sites: 3
pi: 0.00118
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	TAATGCCTACAGTAGAAGGG	GGGGGCGAAGTGATACAAAA	45.0	50.0	53.5	58.2	1357-1360 bp	1.0	43.3
atpF_p2	ATAATGCCTACAGTAGAAGGG	GGGGGCGAAGTGATACAAAA	42.9	50.0	54.2	58.2	1358-1361 bp	1.0	40.6
atpF_p3	AATAATGCCTACAGTAGAAGGG	GGGGGCGAAGTGATACAAAA	40.9	50.0	55.0	58.2	1359-1362 bp	1.0	40.4
atpF_p4	AAATAATGCCTACAGTAGAAGGG	GGGGGCGAAGTGATACAAAA	39.1	50.0	55.8	58.2	1360-1363 bp	1.0	40.4
atpF_p5	TAATGCCTACAGTAGAAGGG	GGGCGAAGTGATACAAAAGG	45.0	50.0	53.5	56.8	1355-1358 bp	1.0	40.4