Hotspot

petA-psbJ

LSC · 67857-68998 · 1142 bp

Back to Endiandra

Pi 0.0118

MarkerSeek score 67.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1142
variable_sites: 27
pi: 0.0118
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGTTTGTTGT	ATTCCGGATTGGGATCATCC	45.0	50.0	58.2	57.1	1277-1284 bp	1.0	73.4
petA-psbJ_p2	ACGTGTCCAAGGTTTGTTGT	ACTGGTATTCCTGTGATCGG	45.0	50.0	58.2	56.7	1326-1333 bp	1.0	70.2
petA-psbJ_p3	ACGTGTCCAAGGTTTGTTGT	CTGGTATTCCTGTGATCGGT	45.0	50.0	58.2	56.7	1325-1332 bp	1.0	70.2
petA-psbJ_p4	ACGTGTCCAAGGTTTGTTGT	GGTTCATATTCCGGATTGGGA	45.0	47.6	58.2	57.5	1284-1291 bp	1.0	68.5
petA-psbJ_p5	ACGTGTCCAAGGTTTGTTGT	ACTGGTATTCCTGTGATCGGT	45.0	47.6	58.2	58.5	1326-1333 bp	1.0	68.2