Hotspot

ndhC-trnV-UAC

LSC · 54024-55866 · 1843 bp

Back to Endiandra

Pi 0.00218
MarkerSeek score 60.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1843
variable_sites
8
pi
0.00218
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CTTCACTAATCGGGGCCAAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1976-1985 bp 1.0 78.2
ndhC-trnV-UAC_p2 ACTAATCGGGGCCAAAACTC AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1972-1981 bp 1.0 78.2
ndhC-trnV-UAC_p3 CACTAATCGGGGCCAAAACT AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.2 57.8 1973-1982 bp 1.0 77.8
ndhC-trnV-UAC_p4 TCACTAATCGGGGCCAAAAC AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.2 57.8 1974-1983 bp 1.0 77.8
ndhC-trnV-UAC_p5 CTTCACTAATCGGGGCCAAA ACGCATGTTGGGTCTTTGAA 50.0 45.0 57.9 58.3 2042-2051 bp 1.0 77.3