Hotspot

ycf3-trnS-GGA

LSC · 48007-48773 · 767 bp

Back to Endiandra

Pi 0.00174

MarkerSeek score 60.00

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 767
variable_sites: 2
pi: 0.00174
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTCTCCTGAAGTTGTCGGAA	TTGTGGTCGGTTGGTGAATT	45.0	45.0	56.4	57.9	980-982 bp	1.0	67.1
ycf3-trnS-GGA_p2	TTTCTCCTGAAGTTGTCGGA	TTGTGGTCGGTTGGTGAATT	45.0	45.0	56.4	57.9	981-983 bp	1.0	67.1
ycf3-trnS-GGA_p3	TTCTCCTGAAGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	950-952 bp	1.0	66.4
ycf3-trnS-GGA_p4	TTTCTCCTGAAGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	951-953 bp	1.0	66.4
ycf3-trnS-GGA_p5	TTCTCCTGAAGTTGTCGGAA	TTCCAATGCTACGCCTTGAA	45.0	45.0	56.4	57.8	900-902 bp	1.0	66.1