Hotspot

petN-psbM

LSC · 31248-32276 · 1029 bp

Back to Endiandra

Pi 0.00113

MarkerSeek score 59.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1029
variable_sites: 2
pi: 0.00113
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	ATTGCTACTGCGCTGTTCAT	50.0	45.0	58.2	58.3	1192-1193 bp	1.0	76.9
petN-psbM_p2	GGGGACACAATTCACATGGA	ATTGCTACTGCGCTGTTCAT	50.0	45.0	57.8	58.3	1214-1215 bp	1.0	76.4
petN-psbM_p3	AGGGGACACAATTCACATGG	ATTGCTACTGCGCTGTTCAT	50.0	45.0	57.8	58.3	1215-1216 bp	1.0	76.4
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TATTGCTACTGCGCTGTTCA	50.0	45.0	58.2	57.6	1193-1194 bp	1.0	76.0
petN-psbM_p5	GGGGACACAATTCACATGGA	TATTGCTACTGCGCTGTTCA	50.0	45.0	57.8	57.6	1215-1216 bp	1.0	75.5