primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	20	20	55.0	55.0	58.1	57.9	0.0	13.4	0.194818	1642	2029	455-455	1.000000	79.5
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.199180	1642	2029	469-469	1.000000	79.4
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	1642	2029	468-468	1.000000	79.4
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	1642	2029	470-470	1.000000	79.4
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	1642	2029	458-458	1.000000	79.4
matK_p1	matK	1	CCACTACACTACTGCATGAGC	CTTGTTCTGACCGTATCGCA	21	20	52.4	50.0	58.5	58.0	0.0	0.0	1.464516	3676	5199	1715-1715	1.000000	68.5
matK_p2	matK	2	CCACTACACTACTGCATGAGC	TTGTTCTGACCGTATCGCAC	21	20	52.4	50.0	58.5	58.3	0.0	0.0	1.750059	3676	5199	1714-1714	1.000000	66.2
matK_p3	matK	3	TCAACCACTACACTACTGCA	CTTGTTCTGACCGTATCGCA	20	20	45.0	50.0	56.1	58.0	0.0	0.0	1.884608	3676	5199	1719-1719	1.000000	65.2
matK_p4	matK	4	ACCACTACACTACTGCATGAG	CTTGTTCTGACCGTATCGCA	21	20	47.6	50.0	57.1	58.0	0.0	0.0	1.885475	3676	5199	1716-1716	1.000000	65.1
matK_p5	matK	5	CACTACACTACTGCATGAGC	CTTGTTCTGACCGTATCGCA	20	20	50.0	50.0	56.0	58.0	0.0	0.0	2.036123	3676	5199	1714-1714	1.000000	63.9
atpA-atpF_p1	atpA-atpF	1	CCCACTTGAAGGACTGTTCC	CTGGGGGCCATGAAAGAAAT	20	20	55.0	50.0	58.1	58.1	0.0	4.4	0.232376	13693	13765	207-210	1.000000	81.2
atpA-atpF_p2	atpA-atpF	2	CCCACTTGAAGGACTGTTCC	AAGGAGCCCTAGGAACTCTG	20	20	55.0	55.0	58.1	58.1	0.0	23.3	0.229667	13693	13765	284-287	1.000000	80.4
atpA-atpF_p3	atpA-atpF	3	CCCACTTGAAGGACTGTTCC	CAAGGAGCCCTAGGAACTCT	20	20	55.0	55.0	58.1	58.1	0.0	23.3	0.229667	13693	13765	285-288	1.000000	80.4
atpA-atpF_p4	atpA-atpF	4	CCCACTTGAAGGACTGTTCC	GGAGCCCTAGGAACTCTGAA	20	20	55.0	55.0	58.1	58.1	0.0	23.3	0.235077	13693	13765	282-285	1.000000	80.4
atpA-atpF_p5	atpA-atpF	5	CCCACTTGAAGGACTGTTCC	TAATATTGGCATGCTGGGGG	20	20	55.0	50.0	58.1	57.7	0.0	18.9	0.409936	13693	13765	220-223	1.000000	79.6
atpF_p1	atpF	1	TAATGCCTACAGTAGAAGGG	GGGGGCGAAGTGATACAAAA	20	20	45.0	50.0	53.5	58.2	9.4	0.0	4.633834	13766	15039	1357-1360	1.000000	43.3
atpF_p2	atpF	2	ATAATGCCTACAGTAGAAGGG	GGGGGCGAAGTGATACAAAA	21	20	42.9	50.0	54.2	58.2	9.4	0.0	4.967994	13766	15039	1358-1361	1.000000	40.6
atpF_p3	atpF	3	AATAATGCCTACAGTAGAAGGG	GGGGGCGAAGTGATACAAAA	22	20	40.9	50.0	55.0	58.2	9.4	0.0	5.139335	13766	15039	1359-1362	1.000000	40.4
atpF_p4	atpF	4	AAATAATGCCTACAGTAGAAGGG	GGGGGCGAAGTGATACAAAA	23	20	39.1	50.0	55.8	58.2	9.4	0.0	5.383064	13766	15039	1360-1363	1.000000	40.4
atpF_p5	atpF	5	TAATGCCTACAGTAGAAGGG	GGGCGAAGTGATACAAAAGG	20	20	45.0	50.0	53.5	56.8	9.4	0.0	5.667765	13766	15039	1355-1358	1.000000	40.4
petN-psbM_p1	petN-psbM	1	TAGTAAGTCTCGCTTGGGCT	ATTGCTACTGCGCTGTTCAT	20	20	50.0	45.0	58.2	58.3	0.0	25.3	0.408587	31248	32276	1192-1193	1.000000	76.9
petN-psbM_p2	petN-psbM	2	GGGGACACAATTCACATGGA	ATTGCTACTGCGCTGTTCAT	20	20	50.0	45.0	57.8	58.3	0.0	25.3	0.471615	31248	32276	1214-1215	1.000000	76.4
petN-psbM_p3	petN-psbM	3	AGGGGACACAATTCACATGG	ATTGCTACTGCGCTGTTCAT	20	20	50.0	45.0	57.8	58.3	0.0	25.3	0.477892	31248	32276	1215-1216	1.000000	76.4
petN-psbM_p4	petN-psbM	4	TAGTAAGTCTCGCTTGGGCT	TATTGCTACTGCGCTGTTCA	20	20	50.0	45.0	58.2	57.6	0.0	25.3	0.529793	31248	32276	1193-1194	1.000000	76.0
petN-psbM_p5	petN-psbM	5	GGGGACACAATTCACATGGA	TATTGCTACTGCGCTGTTCA	20	20	50.0	45.0	57.8	57.6	0.0	25.3	0.592821	31248	32276	1215-1216	1.000000	75.5
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	GAAACGGTCCCTCCTTAACC	20	20	55.0	55.0	58.1	57.9	7.6	0.0	0.169074	35039	36426	1530-1530	1.000000	78.9
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	GAAACGGTCCCTCCTTAACC	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.191228	35039	36426	1533-1533	1.000000	78.7
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	20	20	55.0	45.0	58.1	58.2	7.6	0.0	0.310137	35039	36426	1538-1538	1.000000	77.7
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.332292	35039	36426	1541-1541	1.000000	77.5
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GTGGTAGAGTAACGCCATGG	CGAAACGGTCCCTCCTTAAC	20	20	55.0	55.0	58.1	58.3	7.6	0.0	0.345672	35039	36426	1531-1531	1.000000	77.4
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	TTCTCCTGAAGTTGTCGGAA	TTGTGGTCGGTTGGTGAATT	20	20	45.0	45.0	56.4	57.9	0.0	0.0	1.644221	48007	48773	980-982	1.000000	67.1
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	TTTCTCCTGAAGTTGTCGGA	TTGTGGTCGGTTGGTGAATT	20	20	45.0	45.0	56.4	57.9	0.0	0.0	1.644221	48007	48773	981-983	1.000000	67.1
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	TTCTCCTGAAGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	20	20	45.0	50.0	56.4	58.2	0.0	0.0	1.735519	48007	48773	950-952	1.000000	66.4
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	TTTCTCCTGAAGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	20	20	45.0	50.0	56.4	58.2	0.0	0.0	1.735519	48007	48773	951-953	1.000000	66.4
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TTCTCCTGAAGTTGTCGGAA	TTCCAATGCTACGCCTTGAA	20	20	45.0	45.0	56.4	57.8	0.0	5.6	1.776834	48007	48773	900-902	1.000000	66.1
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CTTCACTAATCGGGGCCAAA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.9	57.8	4.4	0.0	0.320066	54024	55866	1976-1985	1.000000	78.2
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	ACTAATCGGGGCCAAAACTC	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.9	57.8	4.4	0.0	0.321560	54024	55866	1972-1981	1.000000	78.2
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CACTAATCGGGGCCAAAACT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.2	57.8	4.4	0.0	0.363095	54024	55866	1973-1982	1.000000	77.8
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TCACTAATCGGGGCCAAAAC	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.2	57.8	4.4	0.0	0.368304	54024	55866	1974-1983	1.000000	77.8
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CTTCACTAATCGGGGCCAAA	ACGCATGTTGGGTCTTTGAA	20	20	50.0	45.0	57.9	58.3	4.4	0.0	0.436429	54024	55866	2042-2051	1.000000	77.3
trnV-UAC_p1	trnV-UAC	1	CCACGCGCACTAATTAGGAA	TGGAATATCCATCTTGACAAGA	20	22	50.0	36.4	58.4	54.6	16.7	19.8	5.760665	55867	56531	753-754	1.000000	40.5
trnV-UAC_p2	trnV-UAC	2	CCACGCGCACTAATTAGGAA	TGGAATATCCATCTTGACAAGAA	20	23	50.0	34.8	58.4	55.4	16.7	21.3	5.979820	55867	56531	753-754	1.000000	40.5
trnV-UAC_p3	trnV-UAC	3	CGTGATCCTGTGGGAATGAT	TGGAATATCCATCTTGACAAGA	20	22	50.0	36.4	57.4	54.6	0.0	19.8	6.035526	55867	56531	803-804	1.000000	40.5
trnV-UAC_p4	trnV-UAC	4	CGTGATCCTGTGGGAATGAT	TGGAATATCCATCTTGACAAGAA	20	23	50.0	34.8	57.4	55.4	0.0	21.3	6.254680	55867	56531	803-804	1.000000	40.5
trnV-UAC_p5	trnV-UAC	5	CCACGCGCACTAATTAGGAA	TGGAATATCCATCTTGACAAGAAA	20	24	50.0	33.3	58.4	56.1	16.7	21.3	6.264765	55867	56531	753-754	1.000000	40.5
petA-psbJ_p1	petA-psbJ	1	ACGTGTCCAAGGTTTGTTGT	ATTCCGGATTGGGATCATCC	20	20	45.0	50.0	58.2	57.1	0.0	4.1	1.058172	67857	68998	1277-1284	1.000000	73.4
petA-psbJ_p2	petA-psbJ	2	ACGTGTCCAAGGTTTGTTGT	ACTGGTATTCCTGTGATCGG	20	20	45.0	50.0	58.2	56.7	0.0	0.0	1.450681	67857	68998	1326-1333	1.000000	70.2
petA-psbJ_p3	petA-psbJ	3	ACGTGTCCAAGGTTTGTTGT	CTGGTATTCCTGTGATCGGT	20	20	45.0	50.0	58.2	56.7	0.0	0.0	1.450681	67857	68998	1325-1332	1.000000	70.2
petA-psbJ_p4	petA-psbJ	4	ACGTGTCCAAGGTTTGTTGT	GGTTCATATTCCGGATTGGGA	20	21	45.0	47.6	58.2	57.5	0.0	4.1	1.673144	67857	68998	1284-1291	1.000000	68.5
petA-psbJ_p5	petA-psbJ	5	ACGTGTCCAAGGTTTGTTGT	ACTGGTATTCCTGTGATCGGT	20	21	45.0	47.6	58.2	58.5	0.0	0.0	1.703141	67857	68998	1326-1333	1.000000	68.2
clpP-psbB_p1	clpP-psbB	1	CTTTTGGAACACCAATGGGC	GCCGACCAGGATCATTCAAT	20	20	50.0	50.0	57.8	58.0	3.6	0.0	0.195454	77085	77522	515-515	1.000000	79.1
clpP-psbB_p2	clpP-psbB	2	CTTTTGGAACACCAATGGGC	ATATGGACAGAAAGCAGCCG	20	20	50.0	50.0	57.8	58.0	3.6	0.0	0.209284	77085	77522	531-531	1.000000	78.9
clpP-psbB_p3	clpP-psbB	3	ACCCAAACTGCATCTTCCTC	GCCGACCAGGATCATTCAAT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.233704	77085	77522	555-555	1.000000	78.7
clpP-psbB_p4	clpP-psbB	4	ACCCAAACTGCATCTTCCTC	ATATGGACAGAAAGCAGCCG	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.247535	77085	77522	571-571	1.000000	78.6
clpP-psbB_p5	clpP-psbB	5	CTTTTGGAACACCAATGGGC	GGCCCAACCAGAAACTAGAG	20	20	50.0	55.0	57.8	57.9	3.6	0.0	0.283669	77085	77522	562-562	1.000000	78.3
rps12-trnV-GAC_p1	rps12-trnV-GAC	1	TGGCAACTTTACGTAAGGCA	CGGGGGAGCAAGTCAAAATA	20	20	45.0	50.0	57.7	57.9	16.5	0.0	0.380474	102316	104103	1945-1950	1.000000	77.2
rps12-trnV-GAC_p2	rps12-trnV-GAC	2	TGGCAACTTTACGTAAGGCA	GTCAAGGTGACACTCTACCG	20	20	45.0	55.0	57.7	57.7	16.5	5.4	0.606988	102316	104103	1869-1874	1.000000	75.4
rps12-trnV-GAC_p3	rps12-trnV-GAC	3	TGGCAACTTTACGTAAGGCA	GGCTCGAACTGATGACTTCC	20	20	45.0	55.0	57.7	58.4	16.5	0.0	0.611502	102316	104103	1894-1899	1.000000	75.4
rps12-trnV-GAC_p4	rps12-trnV-GAC	4	TGGCAACTTTACGTAAGGCA	GCTCGAACTGATGACTTCCA	20	20	45.0	50.0	57.7	57.6	16.5	0.0	0.627972	102316	104103	1893-1898	1.000000	75.2
rps12-trnV-GAC_p5	rps12-trnV-GAC	5	TGGCAACTTTACGTAAGGCA	CAGGCTCGAACTGATGACTT	20	20	45.0	50.0	57.7	57.6	16.5	10.4	0.634499	102316	104103	1896-1901	1.000000	75.2
ndhF-rpl32_p1	ndhF-rpl32	1	CTGGAAGTGGAGCAAAAGGT	TGACATCCCTTTCCCTTCCA	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.259215	117101	118291	1313-1322	1.000000	78.8
ndhF-rpl32_p2	ndhF-rpl32	2	CGGAACAAACAGAAGTCCCA	TGACATCCCTTTCCCTTCCA	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.284304	117101	118291	1351-1360	1.000000	78.6
ndhF-rpl32_p3	ndhF-rpl32	3	CTGGAAGTGGAGCAAAAGGT	GTACGTTTCTTTGGAACCGC	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.318370	117101	118291	1259-1268	1.000000	78.4
ndhF-rpl32_p4	ndhF-rpl32	4	CGGAACAAACAGAAGTCCCA	GTACGTTTCTTTGGAACCGC	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.343458	117101	118291	1297-1306	1.000000	78.2
ndhF-rpl32_p5	ndhF-rpl32	5	CTGGAAGTGGAGCAAAAGGT	CTGACATCCCTTTCCCTTCC	20	20	50.0	55.0	58.0	57.6	0.0	0.0	0.439960	117101	118291	1314-1323	1.000000	77.4
ndhD_p1	ndhD	1	TTGCATTGTACAGTGAACGA	ACCGACAAAAACCCGTACTC	20	20	40.0	50.0	55.6	58.1	21.3	0.0	2.499788	121197	122702	1655-1655	1.000000	60.1
ndhD_p2	ndhD	2	TTGCATTGTACAGTGAACGA	GCGGGTTTTTCTGGTCAAAG	20	20	40.0	50.0	55.6	57.9	21.3	0.0	2.503763	121197	122702	1618-1618	1.000000	60.1
ndhD_p3	ndhD	3	CATTGCATTGTACAGTGAACGA	ACCGACAAAAACCCGTACTC	22	20	40.9	50.0	57.6	58.1	21.3	0.0	2.533241	121197	122702	1657-1657	1.000000	59.8
ndhD_p4	ndhD	4	TCATTGCATTGTACAGTGAACG	ACCGACAAAAACCCGTACTC	22	20	40.9	50.0	57.6	58.1	21.3	0.0	2.533241	121197	122702	1658-1658	1.000000	59.8
ndhD_p5	ndhD	5	CATTGCATTGTACAGTGAACGA	GCGGGTTTTTCTGGTCAAAG	22	20	40.9	50.0	57.6	57.9	21.3	0.0	2.537216	121197	122702	1620-1620	1.000000	59.8
ndhG-ndhI_p1	ndhG-ndhI	1	GTAATACCACCCCCAGACCT	TCAAATTGCTTTGGGTCGAT	20	20	55.0	40.0	58.1	55.9	0.0	0.0	2.189547	124423	124815	545-550	1.000000	63.7
ndhG-ndhI_p2	ndhG-ndhI	2	AGTAATACCACCCCCAGACC	ATCAAATTGCTTTGGGTCGA	20	20	55.0	40.0	58.1	55.9	0.0	0.0	2.189547	124423	124815	547-552	1.000000	63.6
ndhG-ndhI_p3	ndhG-ndhI	3	AGTAATACCACCCCCAGACC	TCAAATTGCTTTGGGTCGAT	20	20	55.0	40.0	58.1	55.9	0.0	0.0	2.189547	124423	124815	546-551	1.000000	63.6
ndhG-ndhI_p4	ndhG-ndhI	4	GTAATACCACCCCCAGACCT	ATCAAATTGCTTTGGGTCGA	20	20	55.0	40.0	58.1	55.9	0.0	0.0	2.189547	124423	124815	546-551	1.000000	63.6
ndhG-ndhI_p5	ndhG-ndhI	5	TAATACCACCCCCAGACCTC	TCAAATTGCTTTGGGTCGAT	20	20	55.0	40.0	57.8	55.9	0.0	0.0	2.264383	124423	124815	544-549	1.000000	63.1
trnV-GAC-rps12_p1	trnV-GAC-rps12	1	CGGGGGAGCAAGTCAAAATA	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.9	57.7	0.0	16.5	0.380474	143826	145613	1945-1950	1.000000	77.2
trnV-GAC-rps12_p2	trnV-GAC-rps12	2	GTCAAGGTGACACTCTACCG	TGGCAACTTTACGTAAGGCA	20	20	55.0	45.0	57.7	57.7	5.4	16.5	0.606988	143826	145613	1869-1874	1.000000	75.4
trnV-GAC-rps12_p3	trnV-GAC-rps12	3	GGCTCGAACTGATGACTTCC	TGGCAACTTTACGTAAGGCA	20	20	55.0	45.0	58.4	57.7	0.0	16.5	0.611502	143826	145613	1894-1899	1.000000	75.4
trnV-GAC-rps12_p4	trnV-GAC-rps12	4	GCTCGAACTGATGACTTCCA	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.6	57.7	0.0	16.5	0.627972	143826	145613	1893-1898	1.000000	75.2
trnV-GAC-rps12_p5	trnV-GAC-rps12	5	CAGGCTCGAACTGATGACTT	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.6	57.7	10.4	16.5	0.634499	143826	145613	1896-1901	1.000000	75.2