Hotspot

rps15

SSC · 121764-122033 · 270 bp

Back to Emilia

Pi 0.502
MarkerSeek score 77.00
Primer available yes
Label rps15

Feature Metadata

label_name
rps15
feature_type
gene
region
SSC
length_bp
270
variable_sites
126
pi
0.502
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15_p1 TGAGGTAAATCAGCAGCGAC AATAATGGGAGCCGTTTGGG 50.0 50.0 58.0 58.2 1118 bp 0.5 58.1
rps15_p2 AACACCATGCATTGATGGGT AATAATGGGAGCCGTTTGGG 45.0 50.0 58.1 58.2 687 bp 0.5 57.7
rps15_p3 CCTAATTGCTCTGGGCCATT AATAATGGGAGCCGTTTGGG 50.0 50.0 57.9 58.2 881 bp 0.5 57.6
rps15_p4 TGGGATTCCGCGTAATAAGC AATAATGGGAGCCGTTTGGG 50.0 50.0 58.1 58.2 1204 bp 0.5 57.2
rps15_p5 GCTCTGGGCCATTTACAGTT AATAATGGGAGCCGTTTGGG 50.0 50.0 58.2 58.2 874 bp 0.5 56.9