Hotspot

psaC

SSC · 115703-115948 · 246 bp

Back to Emilia

Pi 0.47
MarkerSeek score 77.10
Primer available yes
Label psaC

Feature Metadata

label_name
psaC
feature_type
gene
region
SSC
length_bp
246
variable_sites
109
pi
0.47
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC_p1 TTTCTTTCCTCCCCACATGC TGGCACTGTCAATCCAGAAG 50.0 50.0 58.1 57.8 976 bp 0.5 57.9
psaC_p2 TTTCTTTCCTCCCCACATGC GGCACTGTCAATCCAGAAGT 50.0 50.0 58.1 57.8 975 bp 0.5 57.9
psaC_p3 GAGACGTTTCTTTCCTCCCC TGGCACTGTCAATCCAGAAG 55.0 50.0 57.9 57.8 982 bp 0.5 57.7
psaC_p4 GAGACGTTTCTTTCCTCCCC GGCACTGTCAATCCAGAAGT 55.0 50.0 57.9 57.8 981 bp 0.5 57.7
psaC_p5 TCCTCCCCACATGCATAGAA TGGCACTGTCAATCCAGAAG 50.0 50.0 58.1 57.8 970 bp 0.5 57.6