Hotspot

ndhI

SSC · 117716-118216 · 501 bp

Pi 0.448

MarkerSeek score 76.50

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 501
variable_sites: 173
pi: 0.448
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	AGTTACTCCATCTCCCACGG	AGCCGAGTGTGAACGATTAC	55.0	50.0	58.5	58.0	1753 bp	0.5	55.8
ndhI_p2	TTACTCCATCTCCCACGGTC	AGCCGAGTGTGAACGATTAC	55.0	50.0	58.5	58.0	1751 bp	0.5	55.8
ndhI_p3	AGTTACTCCATCTCCCACGG	GGTTCTGCAATAGGGATGGG	55.0	55.0	58.5	58.0	1915 bp	0.5	55.8
ndhI_p4	TTACTCCATCTCCCACGGTC	GGTTCTGCAATAGGGATGGG	55.0	55.0	58.5	58.0	1913 bp	0.5	55.8
ndhI_p5	AGTTACTCCATCTCCCACGG	TTTGTGACCTTTGAGGAGCC	55.0	50.0	58.5	58.0	1959 bp	0.5	55.7