Hotspot

ndhG

SSC · 116735-117265 · 531 bp

Back to Emilia

Pi 0.441
MarkerSeek score 76.90
Primer available yes
Label ndhG

Feature Metadata

label_name
ndhG
feature_type
gene
region
SSC
length_bp
531
variable_sites
179
pi
0.441
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.76
markerseek_score
76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG_p1 CTTCTGGATTGACAGTGCCA ATTTGCCCGTTGTTGATTGG 50.0 45.0 57.8 57.5 1919 bp 0.5 54.9
ndhG_p2 ACTTCTGGATTGACAGTGCC ATTTGCCCGTTGTTGATTGG 50.0 45.0 57.8 57.5 1920 bp 0.5 54.8
ndhG_p3 CTTCTGGATTGACAGTGCCA TGCCCGTTGTTGATTGGAAA 50.0 45.0 57.8 58.6 1916 bp 0.5 53.7
ndhG_p4 CTTCTGGATTGACAGTGCCA TTGCCCGTTGTTGATTGGAA 50.0 45.0 57.8 58.6 1917 bp 0.5 53.7
ndhG_p5 CTTCTGGATTGACAGTGCCA TTTGCCCGTTGTTGATTGGA 50.0 45.0 57.8 58.6 1918 bp 0.5 53.7