Hotspot

ndhF

SSC · 108748-110970 · 2223 bp

Back to Emilia

Pi 0.449
MarkerSeek score 80.70
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2223
variable_sites
899
pi
0.449
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.90
markerseek_score
80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 CTAGCACTGGGTAGACCTCA AGGTAAAATCCGGTGAGTCG 55.0 50.0 57.9 57.3 2642 bp 0.5 53.5
ndhF_p2 TCGTTGTGGTCGGACTCTAT AGGTAAAATCCGGTGAGTCG 50.0 50.0 58.2 57.3 2821 bp 0.5 53.3
ndhF_p3 ATCAATTCGGTCGTTGTGGT AGGTAAAATCCGGTGAGTCG 45.0 50.0 57.8 57.3 2831 bp 0.5 53.2
ndhF_p4 TCTCTTCCTTCTCCGAGCTC AGGTAAAATCCGGTGAGTCG 55.0 50.0 58.2 57.3 2763 bp 0.5 52.7
ndhF_p5 TGTTTCGATGCAACAGCAAG AGGTAAAATCCGGTGAGTCG 45.0 50.0 57.6 57.3 2416 bp 0.5 51.4