Hotspot

ndhD

SSC · 114087-115607 · 1521 bp

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Pi 0.49
MarkerSeek score 81.00
Primer available yes
Label ndhD

Feature Metadata

label_name
ndhD
feature_type
gene
region
SSC
length_bp
1521
variable_sites
634
pi
0.49
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.85
markerseek_score
81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD_p1 GGGCTCATATTGGAGTTGGG GGGACGGATGTAAAGCCAAA 55.0 50.0 58.0 58.2 2282 bp 0.5 58.5
ndhD_p2 GGGGCTCATATTGGAGTTGG GGGACGGATGTAAAGCCAAA 55.0 50.0 58.0 58.2 2283 bp 0.5 58.5
ndhD_p3 GGGCTCATATTGGAGTTGGG TGGCACTGTCAATCCAGAAG 55.0 50.0 58.0 57.8 2513 bp 0.5 58.4
ndhD_p4 GGGGCTCATATTGGAGTTGG TGGCACTGTCAATCCAGAAG 55.0 50.0 58.0 57.8 2514 bp 0.5 58.4
ndhD_p5 GGGCTCATATTGGAGTTGGG GGCACTGTCAATCCAGAAGT 55.0 50.0 58.0 57.8 2512 bp 0.5 58.4