Hotspot

ndhA

SSC · 118295-120492 · 2198 bp

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Pi 0.485
MarkerSeek score 80.00
Primer available yes
Label ndhA

Feature Metadata

label_name
ndhA
feature_type
gene
region
SSC
length_bp
2198
variable_sites
925
pi
0.485
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.87
markerseek_score
80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhA_p1 TGACCAATATACCTTGCGGC AGAGTTGAAGCACCAAAGGG 50.0 50.0 58.0 58.0 2546 bp 0.5 59.6
ndhA_p2 TGACCAATATACCTTGCGGC AGTTGAAGCACCAAAGGGAG 50.0 50.0 58.0 58.0 2544 bp 0.5 59.6
ndhA_p3 TGACCAATATACCTTGCGGC ACCAATGCTACGAGCTTCTG 50.0 50.0 58.0 58.0 2868 bp 0.5 59.6
ndhA_p4 TGACCAATATACCTTGCGGC GAGTTGAAGCACCAAAGGGA 50.0 50.0 58.0 58.0 2545 bp 0.5 59.5
ndhA_p5 TGACCAATATACCTTGCGGC GTTGAAGCACCAAAGGGAGA 50.0 50.0 58.0 58.0 2543 bp 0.5 59.5