Hotspot

ndhG-ndhI

SSC · 117266-117715 · 450 bp

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Pi 0.472
MarkerSeek score 79.80
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
450
variable_sites
196
pi
0.472
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 AGACCACGGAAGTTACTCCA GATGGACTTTACCGAGGCTG 50.0 55.0 58.0 58.1 1458 bp 0.5 59.5
ndhG-ndhI_p2 AGACCACGGAAGTTACTCCA TTGATCACATCCGAGCGTTT 50.0 45.0 58.0 57.9 1123 bp 0.5 59.2
ndhG-ndhI_p3 AGACCACGGAAGTTACTCCA AGATGGACTTTACCGAGGCT 50.0 50.0 58.0 58.1 1459 bp 0.5 58.9
ndhG-ndhI_p4 AGACCACGGAAGTTACTCCA TTACCGAGGCTGAGAATGGA 50.0 50.0 58.0 58.1 1450 bp 0.5 58.8
ndhG-ndhI_p5 AGACCACGGAAGTTACTCCA ATTGATCACATCCGAGCGTT 50.0 45.0 58.0 57.7 1124 bp 0.5 57.4