Hotspot

ndhF-rpl32

SSC · 110971-112131 · 1161 bp

Back to Emilia

Pi 0.429
MarkerSeek score 81.90
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1161
variable_sites
441
pi
0.429
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.89
markerseek_score
81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CGCCCACACGATTAGTTACA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.0 58.0 2258 bp 0.5 59.9
ndhF-rpl32_p2 CGCCCACACGATTAGTTACA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.0 58.0 2259 bp 0.5 59.9
ndhF-rpl32_p3 GTTGCCGTCGGAAAAAGAAG AGCGTGTCTACCGATTTCAC 50.0 50.0 58.0 58.0 1800 bp 0.5 59.7
ndhF-rpl32_p4 GTTGCCGTCGGAAAAAGAAG CAGCGTGTCTACCGATTTCA 50.0 50.0 58.0 58.0 1801 bp 0.5 59.7
ndhF-rpl32_p5 TGCCGTCGGAAAAAGAAGAA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.0 58.0 1798 bp 0.5 59.6