Hotspot

ycf1

SSC · 125174-130813 · 5640 bp

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Pi 0.0428

MarkerSeek score 63.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5640
variable_sites: 241
pi: 0.0428
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CAGTTTCCATTCCCCCAACA	TAAGTGGGCCTTATCGTGGA	50.0	50.0	58.0	58.1	1308-1320 bp	1.0	83.2
ycf1_p2	CAGTTTCCATTCCCCCAACA	AGAAAACGTGGCAGAGGATC	50.0	50.0	58.0	57.9	845 bp	0.5	59.1
ycf1_p3	GTTTCCATTCCCCCAACAGT	AGAAAACGTGGCAGAGGATC	50.0	50.0	58.0	57.9	843 bp	0.5	59.1
ycf1_p4	CAGTTTCCATTCCCCCAACA	GAAGAAAACGTGGCAGAGGA	50.0	50.0	58.0	58.1	847 bp	0.5	59.0
ycf1_p5	GTTTCCATTCCCCCAACAGT	GAAGAAAACGTGGCAGAGGA	50.0	50.0	58.0	58.1	845 bp	0.5	59.0