Hotspot

ndhD-psaC

SSC · 118605-118710 · 106 bp

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Pi 0.0943

MarkerSeek score 77.40

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 106
variable_sites: 10
pi: 0.0943
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	GAACCCGCGAATATCGGTAA	AAACAACTCGAAGCATGGGT	50.0	45.0	57.9	58.0	212 bp	1.0	82.5
ndhD-psaC_p2	GAACCCGCGAATATCGGTAA	GGCATGAAACAACTCGAAGC	50.0	50.0	57.9	58.0	218 bp	1.0	82.3
ndhD-psaC_p3	AAAGAACCCGCGAATATCGG	AAACAACTCGAAGCATGGGT	50.0	45.0	58.4	58.0	215 bp	1.0	80.2
ndhD-psaC_p4	AAAGAACCCGCGAATATCGG	GGCATGAAACAACTCGAAGC	50.0	50.0	58.4	58.0	221 bp	1.0	80.0
ndhD-psaC_p5	GAACCCGCGAATATCGGTAA	TCGAAGCATGGGTCTAGGTT	50.0	50.0	57.9	58.4	205 bp	1.0	79.3