Hotspot

rpl32-trnL-UAG

SSC · 114640-115696 · 1057 bp

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Pi 0.0604
MarkerSeek score 79.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1057
variable_sites
62
pi
0.0604
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CAAAAGGCTTTTTGTACGACA AGCGTGTCTACCGATTTCAC 38.1 50.0 55.6 58.0 1089-1112 bp 1.0 59.1
rpl32-trnL-UAG_p2 CAAAAGGCTTTTTGTACGACA CAGCGTGTCTACCGATTTCA 38.1 50.0 55.6 58.0 1090-1113 bp 1.0 59.1
rpl32-trnL-UAG_p3 CAAAAGGCTTTTTGTACGACA TCCTAAGAGCAGCGTGTCTA 38.1 50.0 55.6 57.9 1099-1122 bp 1.0 58.1
rpl32-trnL-UAG_p4 CAAAAGGCTTTTTGTACGACA CCTAAGAGCAGCGTGTCTAC 38.1 55.0 55.6 57.8 1098-1121 bp 1.0 57.6
rpl32-trnL-UAG_p5 CAAAAGGCTTTTTGTACGACA CTAAGAGCAGCGTGTCTACC 38.1 55.0 55.6 57.8 1097-1120 bp 1.0 57.6