Hotspot

ndhF-rpl32

SSC · 113544-114486 · 943 bp

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Pi 0.0574
MarkerSeek score 74.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
943
variable_sites
41
pi
0.0574
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.76
markerseek_score
74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AGCGTAATTAAAGGTAAAATCCATGA AGCTTTCAACGCTACCCAAT 30.8 45.0 57.5 57.8 874-1083 bp 1.0 47.4
ndhF-rpl32_p2 AGCGTAATTAAAGGTAAAATCCATGA AAAGCTTTCAACGCTACCCA 30.8 45.0 57.5 58.0 876-1085 bp 1.0 47.3
ndhF-rpl32_p3 AGCGTAATTAAAGGTAAAATCCATGA AAGCTTTCAACGCTACCCAA 30.8 45.0 57.5 58.0 875-1084 bp 1.0 47.3
ndhF-rpl32_p4 AGCGTAATTAAAGGTAAAATCCA AAAGCTTTCAACGCTACCCA 30.4 45.0 54.3 58.0 876-1085 bp 1.0 47.3
ndhF-rpl32_p5 AGCGTAATTAAAGGTAAAATCCA AAGCTTTCAACGCTACCCAA 30.4 45.0 54.3 58.0 875-1084 bp 1.0 47.3