Hotspot

petL-petG

LSC · 67271-67444 · 174 bp

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Pi 0.0479

MarkerSeek score 75.20

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 174
variable_sites: 8
pi: 0.0479
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	GGTCTGACCAAGATACGACT	GCAGTTACGAATAATCCAGCC	50.0	47.6	56.4	57.1	268-275 bp	1.0	56.4
petL-petG_p2	GGTCTGACCAAGATACGACTT	GCAGTTACGAATAATCCAGCC	47.6	47.6	57.1	57.1	268-275 bp	1.0	54.4
petL-petG_p3	ACCTCAGCTCTGTTTATTGGTC	GCAGTTACGAATAATCCAGCC	45.5	47.6	58.1	57.1	286-293 bp	1.0	52.7
petL-petG_p4	AGCTCTGTTTATTGGTCTGACC	GCAGTTACGAATAATCCAGCC	45.5	47.6	58.1	57.1	281-288 bp	1.0	52.7
petL-petG_p5	ACCTCAGCTCTGTTTATTGGT	GCAGTTACGAATAATCCAGCC	42.9	47.6	56.9	57.1	286-293 bp	1.0	52.5