Hotspot

ycf4-cemA

LSC · 62092-63152 · 1061 bp

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Pi 0.0462

MarkerSeek score 72.90

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 1061
variable_sites: 48
pi: 0.0462
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GTCGCATCCTACTCCGATTC	CTTCCCTATCCACGATGCTG	55.0	55.0	57.9	57.8	1886-1906 bp	1.0	82.2
ycf4-cemA_p2	TTGGCCTATTTCTTGCGTGT	CTTCCCTATCCACGATGCTG	45.0	55.0	58.1	57.8	1710-1730 bp	1.0	82.2
ycf4-cemA_p3	TGGGGTTTTTGTTGGTTGGA	CTTCCCTATCCACGATGCTG	45.0	55.0	58.0	57.8	2129-2149 bp	1.0	81.9
ycf4-cemA_p4	GTCGCATCCTACTCCGATTC	ACTTCCCTATCCACGATGCT	55.0	50.0	57.9	58.2	1887-1907 bp	1.0	81.9
ycf4-cemA_p5	TGGGGTTTTTGTTGGTTGGA	ACTTCCCTATCCACGATGCT	45.0	50.0	58.0	58.2	2130-2150 bp	1.0	81.5