Hotspot

psaI-ycf4

LSC · 61143-61542 · 400 bp

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Pi 0.063

MarkerSeek score 74.70

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 400
variable_sites: 25
pi: 0.063
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	AGTATTTCCGGCAATTGCGA	CGAGACCCCGCTAGAAATTC	45.0	55.0	58.3	58.1	514-517 bp	1.0	81.1
psaI-ycf4_p2	AGTATTTCCGGCAATTGCGA	TCGAGACCCCGCTAGAAATT	45.0	50.0	58.3	58.5	515-518 bp	1.0	78.2
psaI-ycf4_p3	AGTATTTCCGGCAATTGCGA	TTCGAGACCCCGCTAGAAAT	45.0	50.0	58.3	58.5	516-519 bp	1.0	78.1
psaI-ycf4_p4	TATTTCCGGCAATTGCGATG	CGAGACCCCGCTAGAAATTC	45.0	55.0	57.2	58.1	512-515 bp	1.0	77.0
psaI-ycf4_p5	GTATTTCCGGCAATTGCGAT	CGAGACCCCGCTAGAAATTC	45.0	55.0	57.2	58.1	513-516 bp	1.0	77.0